橘叶中橙皮苷的含量测定

Determination of Hesperidin in Folium Citrus reticulatae

目的：建立药材橘叶含量测定方法。方法：采用HPLC，色谱柱为C18柱，流动相为乙腈-水（20：80），检测波长为283nm。结果：橙皮苷在0．1216—1．216μg线性关系良好，平均回收率99．5％，RSD=0．14％（n=6）。结论：方法简便、准确、重现性好，可作为橘叶药材含量测定方法。

Objective： To establish a method for determination of hesperidin in Folium Citrus reticulatae by HPLC. Method： Hesperidin was separated through C,s column with acetonitrile -water （20：80） as a mobile phase, the detection wavelength was at 283nm. Results： The linear range was 0. 121 6- 1. 216μg. The average recovery was 99.5% with RSD 0. 14%. Conclusion： The method is simple, accurate and rapid with good reproducibility. It can be used as determination of hesperidin in Folium Citrus reticulatae.