淫羊藿苷对人牙周膜细胞增殖和骨保护素mRNA表达的影响

Icariin enhances the proliferation and expression of osteoprotegerin of human periodontal ligament cells in vitro

目的：研究淫羊藿苷对人牙周膜细胞（periodontal ligament cells，PDLCs）增殖和骨保护素（osteoprotegerin，OPG）mRNA表达的影响。方法：体外培养人PDLCs，用MTT法检测不同浓度的淫羊藿苷（0．001、0．01、0、1μg／mL）、不同时间（24、48、72、96h）作用下人PDLCs的增殖水平；RT-PCR检测OPG mRNA的表达。结果：淫羊藿苷（0、001-0．1μg／mL）对人PDLCs增殖和OPG mRNA表达具有促进作用（P〈0．01），0．01μg／mL浓度作用最明显。结论：淫羊藿苷能够促进人PDLCs增殖和OPG mRNA表达。

AIM：To investigate the effects of icariin on the proliferation and expression of osteoprotegefin （OPG） mRNA of the human periodontal ligament cells （HPDLC）. METHODS： HPDLC were cultured in vitro and stimulated with icariin winh different concentrations （0. 001 - 10 μg/mL） for 0,24,48,72 and 96 hours. The ability of proliferation of HPDLC was detected by MTT method and RT - PCR was used to examine the expression of OPG mRNA in HPDLC. RESULTS： The proliferation and expression of OPG mRNA of HPDLC were stimulated by icafiin （ P 〈 0.05 ）, and the most efficient concentration was 0. 1 μg/mL （ P 〈 0.01 ）. CONCLUSION ： Icariin may upregulate the expression of OPG mRNA in HPDLC by stimulating its proliferation.