纳米硒对肉鸡肝细胞中细胞谷胱甘肽过氧化物酶活性的影响

Effect of Nano-selenium on Cellular Glutathione Peroxidase Activity in Chick Hepatocytes

以亚硒酸钠和蛋氨酸硒为对照,研究了纳米单质硒(纳米硒)对肉鸡肝细胞中细胞谷胱甘肽过氧化物酶(cGPx)活性的影响。每种硒源分别以0.01、0.05、0.10、0.30、0.50、1.0μmol/L6个硒添加浓度培养肉鸡肝细胞,测定培养后0、24、48、72、96h肉鸡肝细胞cGPx活性。结果显示:亚硒酸钠添加浓度(以硒计)在0.01￣0.10μmol/L、蛋氨酸硒和纳米硒添加浓度(以硒计)在0.01￣0.30μmol/L,cGPx活性随着硒添加浓度的增加而增加;亚硒酸钠添加浓度在0.10￣1.0μmol/L、蛋氨酸硒添加浓度在0.30￣1.0μmol/L,cGPx活性随着硒添加浓度的增加而下降,而纳米硒添加浓度在0.30￣1.0μmol/L,cGPx活性始终保持在高峰平台。结果表明,3种硒源的剂量-效应关系曲线中的最适剂量范围宽度依次为:纳米硒>蛋氨酸硒>亚硒酸钠。

A new selenium source, nano elemental selenium （Nano-Se） was used to study the effect on cellular glutathione peroxidase （cGPx） in chick hepatocytes, and sodium selenite （Na2SeO3） and seleno-1-methionine （Se-Met） were used as controls. Se was added to the chick hepatocytes at 0.01, 0.05, 0.1, 0.3, 0.5 and 1.0 μmol/L Se, respectively. The results showed that the cGPx activity was increased with elevation of Se concentrations in the range of 0.01-0.10 μmol/L for Na2SeO3 and 0.01-0.30μmol/L for Se-Met or Nano-Se, respectively. The cGPx activity was decreased with elevation of Se concentrations in the range of 0.10-1.0 μmol/L for Na2SeO3 and 0.30-1.0 μmol/L for Se-Met, respectively. However, the cGPx activity still remained steady at a range of 0.30-1.0 μmol/L for NanoSe. The results implicated that the ranking of width range of the most suitable Se concentration for nutrition curve of the three Se forms was： Nano-Se〉Se-Met〉Na2SeO3.