核转录因子ARNT对KLF4调控作用的初步研究

Preliminary Study of ARNT Regulation on KLF4

目的 探讨核转录因子ARNT和KLF4间可能存在的信号调控机制。方法构建ARNT真核表达质粒，并与其二聚体配体基因AHR表达质粒分别联合含KLF4启动子的荧光素酶报告载体体外共转染细胞，通过对荧光素酶活性检测分析ARNT对KLF4基因的调控作用。结果同空白对照比较，细胞中转染含ARNT质粒后，引起KLF4启动子荧光素酶活性增高2倍多。另一方面，共转染ARNT及其二聚体配体基因AHR，KLF4启动子荧光素酶活性较空白对照降低近1／8。结论ARNT可活化KLF4启动子，激活KLF4表达，而AHR共转染明显抑制KLF4启动子活性。

Objective The aim of study is to explore the signal regulation pathway between Krüppel - like Factor 4 （KLF4） and aryl hydrocarbon nuclear translocator factor （ARNT）. Methods ARNT plasmid was constructed and cotransfected in vitro with KLF4-1uciferase reporter vector as well as AHR vector. Results The results of luciferase assay revealed that ARNT vector transfection could increase value of KLF4 to 2 folds, while cotransfected AHR vector could decrease luciferase activities to 8 times greatly. Conclusion ARNT could enhance activity of Klf4 promoter, while AHR compete to combine and antagonize Klf4 promoter activation.