软组织平滑肌肉瘤组织中骨桥蛋白的表达及意义

Detection of osteopontin expression in leiomyosarcoma of soft tissue using tissue microarray technique

目的探讨软组织平滑肌肉瘤(le iomyosarcom a,LMS)中骨桥蛋白的表达情况及意义。方法选取20例LMS,12例平滑肌瘤及9例正常平滑肌组织构建成组织芯片(其中5例LMS为外院病例,未参与构建),应用免疫组化SP法检测骨桥蛋白(osteopontin,OPN)的表达情况。结果20例LMS中18例(90%)OPN呈过表达。12例平滑肌瘤中3例(25%)OPN呈过表达。正常平滑肌组织OPN均为阴性或弱阳性表达。在LMS和平滑肌瘤间,LMS与正常平滑肌组织间OPN过表达率差异均具有显著性(P<0.01);良性组与正常平滑肌组OPN过表达率差异无显著性(P>0.05)。5例发生浸润和(或)转移的OPN均为过表达,且3例为强阳性。结论OPN过表达是LMS发生发展过程中一个常见的分子事件,与其发病机制密切相关。LMS与平滑肌瘤间OPN过表达率的差异,有助于临床上平滑肌肿瘤的鉴别诊断。

Purpose To investigate the significance of osteopontin expression in leiomyosarcoma of human soft tissue. Methods Using tissue microarray and immunohistochemical SP techniques to analyze the osteopontin expression in 20 cases of leiomyosareomas, 12 leiomyomas and 9 normal smooth muscle tissues. Results The overexpression rate of osteopontin in LMS was 90% （ 18/20）, and that in leiomyomas was 25% （3/12） ,whereas the expression of osteopontin in normal smooth muscle tissues was all faintly positive or negative. Overexpression rate of osteopontin had statistical difference between LMS and leiomyomas, LMS and normal smooth muscle tissues （P 〈0.01 ）, but no statistical difference between leiomyomas and normal smooth muscle tissues. All of the 5 LMS that were found to have had invasion and （or） metastasis showed osteopontin overexpression,in which 3 of them exhibited strongly positive. Conclusions Overexpression of osteopontin is a frequent molecule event and highly related to the pathogenesis in LMS. Difference of osteopontin expression between LMS and leiomyomas may help in their differential diagnosis.