高效液相色谱法测定藏药独一味中木犀草素及异鼠李素的含量

Determination of luteolin and isorhamnetin in Herba Lamiophlomis by HPLC

目的建立独一味药材中木犀草素、异鼠李素的含量测定方法。方法采用HPLC法，伊利特-C18柱，以甲醇-水（50：50），磷酸调节pH值至3．0，流速：0．8mL·min^-1，检测波长：360nm。结果木犀草素在7．072～35．360μg·mL^-1，异鼠李素在1．658～8．290μg·mL^-1范围内呈良好的线性关系，r木=0．9997，r异=0．9992。平均回收率分另4为97．8％、98．1％，RSD分别为2．0％、2．5％。结论本法专属性强，灵敏度高，重现性好，适于黄酮类成分的分离与测定。

OBJECTIVE To establish a method for determination of luteolin and isorhamnetin in Herba Lamiophlomis. METHODS Yihte-C18 Column was used,the mobile phase was consisted of methanol- water （50： 50） , adjusted pH to 3.0 with H3 PQ, the flow rate was 0.8mL min^-1 and detection wavelength was set at 360 nm. RESULTS The linear range of luteolin and isorhamnetin were 7. 072 - 35. 360μg · mL^-1, （r = 0.9997） and 1. 658 - 8. 290μg · mL^-1（ r = 0. 9992）, the average recovery and RSD value of luteolin and isorhamnetin were 97.8% ,2.0%, and 98.1% ,2.5%. CONCLUSION This method is sensitive, accurate and can be used for the quality study of Herba Lamiophlomis.