多重PCR分析方法应用于转基因农作物的检测

Analysis Method of Genetically Modified Detection of Multiplex Polymerase Chain Reaction(MPCR)

以转基因大豆、水稻等样品为材料,采用多重PCR技术对转基因作物中常见的启动子、终止子、筛选标记基因和转入的目的基因等多个外源转基因元件进行检测,以期建立一套快速、准确、高效的转基因农作物筛查鉴定技术。通过多重扩增实验,建立了关于转基因大豆检测的大豆内源Lectin基因,花椰菜花叶病毒(Cauliflower mosaic virus,CaMV)35S启动子和根癌农杆菌胭脂碱合成酶基因NOS终止子的三重PCR分析体系,及关于转基因水稻检测的CaMV35S、NPTII和HPT基因的三重PCR分析的技术体系。并以大豆、水稻等农作物样品为检测材料,采用单一PCR和多重PCR技术同时进行检测,结果表明多重PCR方法具有快速、高效、简便、准确等特点,在转基因成分的检测上具有非常重要的实际应用价值和潜力。

The genetically modified ingredients introduced including promoter, terminator, selectable marker genes and structural genes（encoding the novel protein） ete were detected with the multiplex PCR technology in order to establish a quick, exact and effective technical system of the screening of genetically modified crops. The triplex PCR of soya inner LECTIN gene, Cauliflower mosaic virus （CaMV）35S promoter and Agro-baeterium tum ef aciens nopaline synthase （NOS） terminator, and the triplex PCR of CaMV35S promoter, NPTII and HIT genes were successfully experimented. The transgenie soya and rice samples were tested with the multiplex and simplex PCR technique, respectively. The results showed the multiplex PCR was quick, effective, simple and exact, and might play an important role in the detection of genetically modified component.