羊驼催乳素受体基因(PRLR)exon10的克隆与序列分析

Cloning and Sequence Analysis on Exon10 of Prolactin Receptor Gene in Alpacas (Lama pacos)

通过氰仿／异戊醇法从羊驼血液和组织中提取羊驼基因组DNA，首次扩增出羊驼PRLR基因（Prolactin Receptor，PRLR）exon10序列（GenBank登录号为DQ206831），并与其它动物相应区域作了同源性比较，结果表明：羊驼PRLR基因exon10的开放阅读框为1133bp，包括1046bp的编码区和87bp的拖尾区；同源性比较显示，羊驼PRLR基因exon10的核苷酸序列与哺乳动物（牛、绵羊、猪、狗、兔、大鼠等）的同源性较高，达80％，氨基酸序列的同源性则≥66％，而与鱼类的同源性则较低，仅为40％～45％。

In order to study exon10 of prolactin receptor gene（PRLR）in alpacas, and to analyze the homology to other mammals, blood samples and tissue from 60 Huacaya alpacas, breed in China Alpacas Base, were taken and their genomic DNAs were extracted by the method of hydroxybenzene / chloroform, and then cloned, sequenced and submitted to GenBank （DQ206831）. The sequence analysis revealed that the alpaca prolactin receptor exon10 spaned 1 133 bp, and included 1 046 bp encoding region and 87 bp untranslated region. And the multiple sequence alignments revealed that the homology of alpacas is 80 % to the other mammals （ such as cow, sheep, pig, dog, rabbit, rat and so on）, the sequence of amnio acid is more than 66%, but it is only 40% to 45% to the fish at the amnio acid level.