HPLC测定海龙中次黄嘌呤及黄嘌呤含量

Determination of hypoxanthine and xanthine in Syngnathus by HPLC

目的 研究海龙中次黄嘌呤、黄嘌呤含量测定的方法。方法 采用Lichrosper C18柱，以甲醇-0．1％HAc（1：99）为流动相，测定波长λ=254nm。结果 次黄嘌呤在5．91～94．56μg·mL^-1范围内，浓度与峰面积线性关系良好（r=0．9999），平均加样回收率为99．22％，RSD为1．25％；黄嘌呤在2．04～32．64μg·mL^-1范围内，浓度与峰面积线性关系良好（r-0．9998），平均加样回收率为98．05％，RSD为1．21％。结论 该法操作简便，结果可靠，为海龙药材的质量研究提供了有效的方法。

Objective To establish a HPLC method for determination of hypoxanthine and xanthine in Syngnathus. Methods A Lichrosper Cls Column was used. The mobile phase was methanol-0.1%HAc. Walvelength was 254nm. Results The linear range of hypoxanthine was within 5. 91～94. 56μg·mL^-1, r=0. 9999, sample recovery rate was 99. 22%, RSD= 1.25%. The linear range of Xanthine was within 2.04～32.64μg·mL^-1 ,r=0. 9998,sample recovery rate was 98.05% ,RSD=1.21%. Conclusion This method has good repeatability and flexibility. It can be used for quality control in production of Syngnathus.