摘要
目的建立蒺藜总皂苷的含量测定方法。方法采用分光光度法,选用菝葜皂苷元(Sarsasa- pogenin)为对照品,通过高氯酸显色,计算蒺藜药材中总皂苷的含量。结果菝葜皂苷元在0.230 4-0.720 0 mg 范围内具有良好的线性关系(γ=0.999 3),平均回收率为98.54%,RSD为1.57%。结论该方法简便、准确、灵敏,重现性良好,适用于蒺藜总皂苷的含量测定。
Objective To determine the content of total saponins in Tribulus terrestis L. Methods UV spectral method was used. The samples or Sarsasapogenin reacted with the perchloric acid. Results There was good linearity( r = 0. 999 3) during the range 0. 230 4 - 0. 7200mg. The average recovery was 98.54 %, RSD was 1.57 % . Conclusion The method is simple, sensitive and accurate, and it can be used as a quantitative analytical method for Tribulus terrestis L.
出处
《实用药物与临床》
CAS
2006年第2期77-78,共2页
Practical Pharmacy and Clinical Remedies
关键词
蒺藜
菝葜皂苷元
总皂苷
分光光度法
Tribulus terrestris L
Sarsasapogenin
Total saponins
UV-spectral method