摘要
目的建立薄层扫描法测定新风胶囊中黄芪甲苷含量的方法。方法采用氟仿-甲醇-水(65: 35:10)10℃以下放置过夜的下层溶液作为展开系统,10％硫酸显色,测定波长λ=510 nm,薄层扫描。结果黄芪甲苷在1．012-5．060 μg范围内线性良好,r=0．999 1,平均回收率为96．7％,RSD=2．18％。结论本法快捷、简便、准确,可用于该制剂质量的控制。
Objective To establish a method for the determination of astragaloside Ⅳ in xinfeng capsule by TLC-scanning. Methods The mobile phase is ch10roform-methanol-water（65 ： 35 ; 10 ） below 10℃, and the plate was scanned at 510nm by the CAMAG Scanner-3. Results The calibration curve was linear in the range of 1. 012 - 5. 060 μg, r = 0. 999 1, the average recovery was 96.7 %, RSD = 2.18 %. Conclusion The method was quick, simple, accurate and suitable for the content determination of astragaloside IV in xinfeng capsule.
出处
《实用药物与临床》
CAS
2006年第2期82-83,共2页
Practical Pharmacy and Clinical Remedies
