RNA干扰技术及其在细胞周期研究中的应用

RNA interference and its application in cell cycle

RNA干扰(RNA interference,RNA i)是由双链RNA(doub le-stranded RNA,dsRNA)引发的转录后基因沉默(posttran-scridptional gene silenc ing,PTGS)。dsRNA经D icer酶降解成21-23nt的siRNA,并以其为模板,特定位点、特定间隔降解与之序列相应的mRNA。随着RNA i机制的深入研究与广泛应用,目前该技术已经普遍应用于细胞周期研究中,在阐明各种调控机制的同时也为基因治疗提供了新靶点。

RNA interference is a mechanism of posttranscriptional gene silencing （PTGS） induced by double - stranded RNA. dsRNA is firstly degradated into 21 -23nt siRNA by Dicer RNase, and then makes itself as a template, triggets sequence -specific mRNA todegradate at the specific site and the specific time. Along with the deep study and the wide application of RNAi, now this strategy has been applied in cell cycle research. It can not only elucidate various mechanisms of cell cycle controlling, but also supply several new targets for gene therapy.