NGF和GM1联合应用对坐骨神经损伤大鼠初级传入神经元的保护作用

Protective effects of NGF and GM1 on primary sensory neurons in SD rat with sciatic nerve injury

目的:探讨联合应用神经营养因子(NGF)和神经节苷脂(GM1)对大鼠周围神经损伤后初级传入神经元的保护作用。方法:选用SD大鼠96只,随机分为对照组、NGF组、GM1组和NGF+GM1组。将大鼠右侧坐骨神经切断,神经两断端相距5 mm,硅胶管桥接,用不同的药物处理后,于术后不同时间取出背根神经节进行研究。结果:4周时神经元数和神经传导速度,NGF+GM1组多于或快于其它各组(P<0.01),NGF组和GM1组均多于或快于对照组(P<0.01);8周时,各实验组多于或快于对照组(P<0.01),各实验组之间差异无统计学意义(P>0.05)。结论:周围神经损伤后,联合应用NGF和GM1对初级传入神经元的保护作用优于单用NGF、GM1,且起效早。

Objective： To observe the protective effects of nerve growth factor （NGF） and mono-siaganglioside （GM1） on primary sensory neurons in rats with sciatic nerve injury. Methods： Ninety-six SD rats were randomly divided into normal saline control group（A）, NGF group（B）, GM1 group（C） and NGF ＋ GM1 group（D）. The sciatic nerve was incised with a 5 nun-defect. The two ends of nerve were connected with a 10.0mm length silicagel tube of 2.0 mm bore. Dorsal root ganglia （DRG） was removed from each animal at different time after treated with saline, NGF, GM1 and NGF＋ GM1 respectively. Results： At the 4th week after operation,the number of neurons and nerve conductive velocity（NCV） increased significantly（P 〈 0.01） in the treatment groups than in the control group, and it increased significantly（P 〈0.01） in group D than that in group B and C. At the 8th week after operation, the number of neurons and NCV increased significantly（ P 〈 0.01 ） in the treatment groups than that in the control group; whereas there were no significant difference between each treatment groups. Condusion： Using NGF and GM1 simultaneously may avoid the necrosis of neurons and accelerate the regeneration of DRG neurons when peripheral nerves are damaged, which is superior to that using NGF or GM1 alone.