慢性乙型病毒性肝炎、肝癌组织HLA-Ⅰ类抗原的表达及意义

Hepatic tissue expression of HLA-I in patients with chronic hepatitis B virus and hepatocellular carcinoma

目的:探讨慢性乙型肝炎、肝癌组织中人类白细胞抗原I(HLA-I)的表达和临床意义。方法:应用流式细胞仪对51例慢性乙型肝炎患者肝组织和43例原发性肝癌癌组织中的HLA-I抗原进行检测。结果:肝癌组织中HLA-I抗原表达为36.47±19.38,明显低于慢性乙肝的79.54±41.55和正常肝组织的81.86±46.53(P均<0.01),而慢性乙型肝炎肝组织和正常肝组织HLA-I类抗原表达无明显差异(P>0.05),伴有肝内和(或)淋巴结转移的肝癌组织中HLA-I抗原表达较无转移组明显降低(22.83±11.59 vs 46.28±23.16,P<0.05);HLA-I类抗原表达与血清AFP水平呈明显负相关(r=-0.741 1,P<0.01),但与肝癌组织分化程度呈明显正相关(r=0.805 1,P<0.05)。结论:HLA-I抗原表达异常与肝癌的发生和转移密切相关。

Objective： To study the expression of human leucocyte antigen Ⅰ（HLA-Ⅰ ） in patients with hepatitis B virus infection and hepatocelhlar carcinoma. Method： HLA-Ⅰ in 51 liver tissues of viral hepatitis B and 43 tumor tissues of hepatocellular carcinoma was examined by flow eytometry. Results： The expression of HLA-Ⅰ was lower in tumor tissue of hepatocelhlar carcinoma（36.47 ± 19.38） than that in liver tissue of chronic viral hepatitis B （79.54 ± 41.55, P 〈 0.01 ） and normal liver tissues（81.86 ± 46.53, P 〈 0.01 ）, but no significant difference was found between the normal liver tissues and tissues of chronic viral hepatitis（ P 〉 0.05）. Moreover, the expression of HLA-Ⅰ in tumor tissues with lymphnode involvement was lower than in tumors without involvement（22.83 ± 11.59 vs 46.28±23.16） （ P 〈0.05）. There was a reverse relation between the expression of HLA-Ⅰ and the level of serum alpha fetoprotein （ r = - 0.741 1, P 〈 0.01 ）, but a positive relation was found between with histological differentiation grading（ r =0.805 1, P 〈0.05）. Conclusion： Low expression of HLA-Ⅰ is relation to development and metastasis of hepatocellular carcinoma.