RT-PCR检测食管癌患者骨髓CEA mRNA的表达及其临床意义

Detection of micrometastasis in the bone marrow of patients with esophageal carcinomas and its clinical implications

目的:探讨食管癌患者骨髓微转移的检测及临床意义。方法:以癌胚抗原(CEA)为分子标记物,采用RT-PCR技术检测43例食管癌患者及17例食管良性病变患者肋骨骨髓中癌胚抗原的表达。结果:43例食管癌患者骨髓中CEA mRNA阳性率为34.9%,食管良性病变患者骨髓中未见表达,两者差异有统计学意义(P<0.05),CEA mRNA的表达与食管癌患者年龄、性别、病理组织类型、肿瘤细胞分化程度无相关性,但与肿瘤浸润深度和淋巴结转移密切相关(P<0.05)。结论:用RT-PCR法检测食管癌患者肋骨骨髓微转移具有较高的敏感性和特异性,骨髓中CEA的检测可帮助综合判断疾病的恶性程度及患者预后,有助于发现亚临床转移灶。

Objective：To study the micrometastasis in the bone marrow of patients with esophageal carcinomas and its clinical implication. Methods： CEA mRNA expression in bone marrow of rib was detected in 43 patients with esophageal carcinoma and 17 patients with esophageal benign lesions as controls by RT-PCR. Results：The positive CEA mRNA expression was 34.9 %（15/43） in bone marrow of patients with esophageal carcinoma and not found in patients with benign lesions （P 〈 0.05）. No correlation was observed between the expression and sex, age, histological type, cancer cell differentiation, but close correlation between the expression and depth of invasion, lymph node metastasis （ P 〈 0.05）. Conclusion： RT-PCR amplification of CEA mRNA is a sensitive and specific method for detection of bone marrow of rib segments in patients with esophageal carcinoma. The detection of CEAmRNA in bone marrow is helpful in comprehensive judging the extent and the prognosis, leading to an early diagnosis and treatment for patients with subclinical metastasis in bone marrow.