γ-干扰素对兔胆总管愈合过程中细胞增殖和转化的影响

Effect of Interferon-γ on cell proliferation and transformation in healing process of bile duct

目的观察γ-干扰素(IFN-γ)对兔胆总管损伤愈合过程中核增殖抗原(PCNA)和α-平滑肌肌动蛋白(α-SMA)表达的影响,为临床防治胆管狭窄寻求一条新的途径。方法制作兔胆总管损伤修复模型16只,随机分为实验组和对照组各8只,实验组每天肌注IFN-γ 10万IU/kg,对照组肌注等体积生理盐水,8周后处死动物,取材,行HE染色,PCNA和α-SMA免疫组化染色,图像分析仪定量分析。结果实验组细胞增殖指数和α-SMA平均阳性染色面积百分比与对照组相比差异均有显著性意义。结论γ-干扰素通过抑制胆管壁PCNA和α-SMA的表达,抑制胆管壁细胞增殖和转化。

Objective To observe the effect of interferon-γ （INF-γ） on the expression of prolifer-ating cell nuclear antigen（PCNA） and α-smooth muscle actin（ α-SMA） in the healing process of the bile duct. Methods Sixteen rabbit bile-duct-injury models were constructed. They were derided into two groups.One was experimental group injected with IFN-γ（100000 IU.kg^-1.d^-1） .The other was control group injected with isotonic Nacl .All rabbits were sacrificed in the eighth week.The expression of PCNA and α-SMA in the anastomotic tissue was observed with immunochemistry and image analysator quantitative analysis. Results There was signifcant deviation between the experimental group and the contral group on the integral optic density per standard area and immunopathology. Conclusion The expression of PCNA and α-SMA can be inhibited by INF-γ. This provides an in vivo basis for INF-γ to inhibit bile duct re-stenosis.