血红素加氧酶-1诱导对鼠肝缺血再灌注损伤的保护作用

Protective role of heme oxygenase-1 on the liver ischemia/reperfusion injury

目的研究血红素加氧酶-1(heme oxygenase-1,HO-1)在鼠肝缺血再灌注损伤肝组织中的表达及其作用。方法建立小鼠部分肝脏热缺血再灌注损伤模型,36只清洁级Balb/C小鼠随机分为3组: 假手术组(S组)、缺血/再灌注损伤组(I/R组)、HO-1诱导剂氯化高铁血红素(hemin)预处理组(HM组)。免疫组化半定量分析肝组织HO-1蛋白的表达,检测血清AST和ALT,肝组织丙二醛(MDA)含量及超氧化物歧化酶(SOD)活性,并观察肝组织的病理变化。结果与S组比较,I/R组HO-1蛋白表达显著增强,hemin预处理后,HO-1蛋白表达较I/R组增高(P<0．01)。I/R组AST,ALT活性和MDA的含量显著高于S组,而HM组均显著低于I/R组(P<0．01);I/R组SOD活性下降,而HM组显著高于I/R组(P<0．01)。HM组病理损伤程度明显轻于I/R组。结论 HO-1在鼠肝缺血再灌注损伤肝组织中表达上调,对肝脏具有保护效应。

Obdective To investigate the protein expression of heme oxygenase-1（HO-1） after liver ischemia/reperfusion（I/R） and its influences. Methods The model of partial hepatic ischemia were established in Balb/C mice. 36 mice were divided at random into sham-operation group（S）, liver ischemia/reperfusion group （I/R）, and induce of HO-1 （heroin） pretreatment group （IN）. The protein expression of HO-1 was observed using immunohistochemical technique and assessed semi-quantltatively. The activity of serum aspartate transaminase （AST） and alanine transaminase（ALT） were assessed. The liver malondialdehyde（NDA） content, SOD activity and liver histpathological changes were detected respectively. ResuIts Compared with that in S group, the expression of HO-1 was significantly increased in I/R group（p〈0.01）. In I/R group, the liver enzymes and MDA content were obviously higher than those in S group （p〈0.01）, but they were decreased obviously when pretreated with heroin, the SOD activity were significantly increased in I/R group, but redued in HM group. Under microscope, the histopathologic results revealed that the injury in HN group was mild compared with that in I/R group. Conclusion The HO-1 protein induced by hemin pretreatment may contribute to the protection on liver I/R injury.