芦丁鼠李糖苷酶基因多位点突变的修复

Rectification of multi-mutation in rutin-α-rhamnosidase gene

任何与原模板不匹配的碱基都可掺入到PCR产物中,形成“不配或错误”。该现象为创造定点突变,基因修复提供了简便易行的方法。利用套叠PCR和高保真DNA聚合酶对芦丁鼠李糖苷酶基因的多个突变位点进行修复,获得100%的修复成功率。

Any alkaline base incompatible to the original module entering into the PCR products generates incompatibility or errors. These phenomena provide simple and easy method for generating definite mutation or gene rectification. Employing overlap extension and high-fidelity thermostable DNA polymerase, the rutin-α-rhamnosidase gene with multiple mutated phases is rectified. The rate of rectification achieves 100%.