人工组织神经移植物引导大鼠再生坐骨神经通过10mm缺损早期的形态学观察

ARTIFICIAL NERVE GRAFTS GUIDE AXONAL REGENERATION ACROSS A 10 mm SCIATIC NERVE GAP IN RATS: MORPHOLOGY OF EARLY OUTGROWTH

本实验采用壳聚糖导管和聚乙醇酸(PGA)纤维支架构成的人工组织神经移植物桥接大鼠10mm坐骨神经缺损,应用免疫荧光组织化学、激光扫描共聚焦显微镜和电镜技术对早期神经再生过程进行观察。结果显示,Schwann细胞和新生轴突沿PGA支架纤维有序地生长。术后4、7、10和14d,新生轴突和Schwann细胞沿支架由近及远长入的距离分别约150μm、500μm、1.3mm和3.5mm。术后4周,新生轴突前沿已经通过整段移植物长到远侧神经端。实验表明,该移植物有利于Schwann细胞和新生轴突的有序导向生长,能够有效地促进周围神经再生。

A novel artificial nerve graft, composed of a chitosan tube and polyglycolic acid （PGA） fibers, was used to bridge a 10 mm gap in rat sciatic nerve, lmmunohistochemistry, laser scanning confocal microscopy and scanning electron microscopy were applied to observe nerve regeneration after implantation. 4, 7, 10 and 14 days postoperatively, outgrowing neurites, mostly accompanied by migrating Schwann cells, extended regularly 150 μm, 500μm, 1.3 mm and 3.5 mm along PGA fibers from the proximal nerve stump to the distal stump, respectively. Four weeks after operation, the pioneers of the regenerating axons had gone through the graft and reached the distal nerve stump. The results indicate that chitosan/PGA artificial nerve grafts may guide regular Schwann cell migration and axonal extension, thus promoting peripheral nerve regeneration effectively.