贝那普利、氨氯地平、卡维地洛对主动脉内皮细胞单层脂类通透性的影响

Effects of Benazepril,Amlodipine Besylate and Carvedilol on Permeability of Aortic Endothelial Cell Monolayers

目的探讨血管紧张素转换酶抑制剂贝那普利、钙离子拮抗剂氨氯地平和β-受体阻滞剂卡维地洛对大鼠主动脉内皮细胞单层通透性的影响。方法分离培养大鼠主动脉内皮细胞(AEC):将AEC培养在混合纤维素酯微孔滤膜上,8 d后形成AEC单层进行通透性测定。将长有AEC的滤膜分别用上述三药处理4 h,置于灌注装置上,用含异硫氰酸荧光素标记白蛋白的高脂血灌注,测定收集液的量和白蛋白、总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白(HDL)、低密度脂蛋白(LDL)浓度。结果贝那普利、氨氯地平能够降低AEC单层对水、TC,TG,HDL,LDL的通透性(P<0.01);氨氯地平降低AEC单层对TC,LDL的通透性更为显著(P<0.001),卡维地洛作用不明显;只有氨氯地平能够降低AEC单层对白蛋白的通透性。结论贝那普利、氨氯地平可减少血浆中脂类渗入血管内皮下,具有潜在的抗动脉粥样硬化作用。

Objective To investigate the effects of angiotensin converting enzyme inhibitor benazepril, calcium channel blocker amlodipine besylate andβ-adrenoceptor agonist carvedilol on the permeability of rat aortic endothelial cell （AEC） monolayers. Methods The method of trauma - free isolation was adopted in the culture of rat AECs. Rat AECs were seeded on the nitrocellulose microporous filters. After an eight days＂ seeding, the monolayers could be used for measuring the permeability. Before being perfused, monolayers were treated with Benazepril, Amlodipine and Carvedilol for four hours successively. The prepared filters were mounted on the Boydon chambers and perfused with hyperlipemia containing fluorescein isothiocyanate （ FITC ） -labeled albumin. The fluid filtering through the monolayers and the filter was collected and the albumin concentration was measured. At the same time, the concentrations of cholesterol, triglyceride （TG） , high-density lipoprotein （HDL） and low-density lipoprotein （LDL） in the collected fluid were also measure by ELISA. Results Benazepril and Amlodipine decreased the permeability of the confluent AEC monolayers to water, cholesterol, TG, HDL and LDL significantly （P 〈 0.01 ）. Amlodipine had more significant effects than the former two drugs. Carvedilol had no obvious effect. Conclusion Benazepril and Amlodipine may decrease the infiltration of lipids and lipoproteins into the subendothelial space, and can be thus used to prevent and ameliorate atherosclerosis.