动脉粥样硬化斑块内新生血管与斑块稳定性之间的关系研究

Influence of endostatin on atherosclerotic plaque stabilization

目的研究内皮抑素对动脉粥样硬化斑块稳定性的影响,评价斑块内新生血管与斑块稳定性之间的关系。方法成年雄性Wistar大鼠建立动脉粥样硬化模型,随机分为两组,分别给予内皮抑素和生理盐水处理,8周后将大鼠处死,测量血脂水平,取主动脉石蜡包埋切片,HE染色,测量斑块面积及内膜中膜面积比;免疫组织化学检测,鼠单克隆抗体CD31培养以计数新生血管数目。结果各模型组血脂水平差异无显著性(P>0.05),均显著高于空白对照组(P<0.05);内皮抑素组内膜面积/中膜面积显著低于模型对照组(P<0.05);内皮抑素组稳定性斑块比例大于模型对照组(P<0.05),新生血管计数内皮抑素组少于模型对照组(P<0.05)。结论内皮抑素能抑制斑块内新生血管生长,抑制斑块进展,增加斑块稳定性。

[Objective] To investigate the influence of endostatin on atherosclerotic plaques. [Methods] To establish the model of atheroselerosis, the rats were randomly divided into 2 groups and treated with endostatin and Nael for 8 weeks respectively. Blood samples were obtained for the analysis of serum cholesterol, the aorta were fixed for histology. [Results] The serum eholesterol level were similar in model group （P 〉0.05）, higher than blank comparison（P 〈0.05）; the amount of neovaseularization were less in endostatin group than in model eomparison（P 〈0.05）. [Conclusion] Endostatin can inhibit neovascularization and plaque growth, promote plaque stabilization.