缺氧诱导因子-1α与上皮型钙黏蛋白在膀胱癌中的表达及相关性研究

Study of expression of HIF-1α and CDH1 in bladder transitional cell carcinoma and their correlation

目的探讨缺氧诱导因子-1α(HIF-1α)、上皮型钙黏蛋白(CDH1)与膀胱移行细胞癌的发生、病理分级和临床分期之间的关系及两者在膀胱移行细胞癌表达的相关性。方法应用免疫组织化学(SP)的方法测定50例膀胱移行细胞癌和12例正常膀胱组织中的HIF-1α与CDH1的表达。结果HIF-1α与膀胱移行细胞癌的发生、病理分化程度和临床分期之间之间明显相关(分别P<0.05、P<0.05、P<0.05),CDH1与膀胱移行细胞癌的发生、病理分化程度和浸润性之间明显相关(分别P<0.01、P<0.05、P<0.01),HIF-1α与CDH1在膀胱移行细胞癌中的表达呈负相关(r=-0.600,P<0.01)。结论HIF-1α与CDH1联合检测可能有助于判断预后,HIF-1α与膀胱移行细胞癌中CDH1的表达下降有关。

[Objective] To investigate association of the expressions of CDH1 and HIF-1α with disease progress, pathological grades and clinic grades in bladder transitional cell carcinoma. And to explore correlation of expressions of CDH1 and HIF-1α in bladder transitional cell carcinoma. [Methods] Expressions of CDH1 and HIF-1α in paraffin-embedded tissue of 50 bladder transitional cell carcinoma and 12 normal population samples were analyzed by immunohistoebemistry staining and consecutive eystectomy. [Results] There was significant correlation between the expression of HIF-1α with disease progress, pathological grades and clinic grades in bladder transitional cell carcinoma （P 〈0.05, P 〈0.05, P 〈0.05 respectively）. There was also significant correlation between the expression of CDH1 with disease progress, pathological grades and clinic grades in bladder transitional cell carcinoma （P 〈0.01, P 〈0.05, P 〈0.01 respectively）. And it was negative correlation between the expression of CDH1 and HIF-1α in bladder transitional cell carcinoma（P 〈0.01）. [Conclusion] Co-detection of HIF-1α and CDH1 may help estimate the prognosis of bladder transitional cell carcinoma. Overexpression of HIF-1α is related to down-regulation of CDH1 in bladder transitional cell carcinoma.