大鼠离体等容收缩心脏模型的研制

The development of isolated isovolumetic contractive heart model in rat

目的在国内首次建立等容收缩离体大鼠心脏模型。方法采用雄性Ｗｉｓｔａｒ大鼠，游离心脏通过主动脉行逆行灌流左心室放入充满生理盐水的乳胶小球，多导生理记录仪（ｓａｎ—ｅｉ３６２）和压力换能器（ＧｏｕｌｄＰ２３ｄｂ）测定血液动力学变化．结果心脏富氧灌流３０ｍｉｎ（ｎ＝６）时，左心室收缩压（ＬＶＳＰ）为９．１±０．３ｋＰａ、左心室舒张压（ＬＶＥＤＰ）ｏｋＰａ、左心室内压最大上升速率（ＬＶｄｐ／ｄｔｍａｘ）１６２．８±６．８ｋＰａ·Ｓ－１心率（ＨＲ）２３７．０±４．０ｂｅａｔｓ·ｍｉｎ－１。结论等容收缩离体大鼠。Ｃ脏模型位测定的心脏血液动力学指标更力。科学与可靠。

The aim of this study was toestablish a isolated heart model of the isovolumetric contraction in rat for the first time in our country .Methods Using male Wistar rats (n= 6),the isolated hearts were perfused retrograde through an aortic cannula and the intraventricular balloon filled with normal saline was used. The cardiac hemodynamics were measured by polygraphy (san-ei 362) and pressure transducer (Gould P23db).Results When isolated rat hearts of isovolumetric contraction were perfused for 30 min , left ventricular systolic pressure (LVSP), left ventricular end-diastolic pressure (LVEDP), and HR were 9. 1± 0.3kPa, 0kPa,162. 8± 6. 8kPa. S-1and 237. 0±4. 0 beats. min-1, respectively.Conclusion The isolated rat heart model of isovolumetric contraction makes the cardiac hemodynamics to be measured much more scientifically and confidently.