HPLC法测定比格犬血浆中西格列羧的血药浓度

HPLC determination of chiglitazar in dog plasma

目的:建立比格犬血浆中西格列羧的定量分析方法,为药物代谢动力学研究提供技术手段。方法:采用高效液相色谱方法,Agilent XDB-C_(18)(4.6 mm×250 mm,5μm)色谱柱,柱温25℃,水(含5%的乙酸)-乙腈(23:77)为流动相,流速1.0 mL·min^(-1),紫外检测波长260 nm,血浆样品经乙腈和乙酸乙酯萃取,以内标法峰面积定量。结果:线性范围为50～2000μg·L^(-1),定量下限为50μg·L^(-1),日内和日间 RSD 在3.8%～11.3%之间,准确度在-0.5%～3.5%之间,本方法涉及的实验条件下样品是稳定的。结论:该方法专属性强,灵敏度高,精密度和准确度好,能够满足药代动力学研究的需要。

Objective： To develop an HPLC assay method for the determination of chiglitazar in dog plasma. Methods： Agilent XDB-C18（4.6 mm×250 mm, 5 μm） column at 25℃ was the stationary phase and water （5% acetic acid） - acetonitrile （23 ： 77） was mobile phase, the flow rate of 1.0 mL·min^-1 and the UV detection wavelength was 260 nm. The plasma samples were extracted with acetonitrile and ethyl acetate. Results： The linearity range was 50 - 2000 μg·L^-1 the limit of quantification was 50 μg·L^-1, the inter - day and intra - day RSD was between 3.8% and 11.3%, the accuracy was between - 0.5% and 3.5% respectively. Conclusion： This assay method is selective, sensitive, precise and accurate, applicable for preclinical pharmacokinetic study.