摘要
目的:建立银屑冲剂中原儿茶酸的含量测定方法。方法:采用反相高效液相色谱法,色谱柱:C_(18)(250 mm×4.6 mm,5 μm);流动相:甲醇-水-冰醋酸(15:85:0.5);流速:1.0 mL.min^(-1);检测波长:260 nm。结果:原儿茶酸在0.1032～0.516μg范围内呈良好线性关系,r=0.99996,平均回收率97.27%,RSD为1.4%。结论:建立的方法简便可行,重现性好,提高了银屑冲剂质量控制方法。
Objective： To set up an assay method for the determination of Yinxie Granules. Methods： The content of Protocatechuic acid was determined by HPLC on a C18 column （250 mm×4. 6 mm, 5 μm）. The mobile phase consisted of CH3OH - H2O - HAc （15：85：0.5）. The flow rate was 1.0 mL. min^-1. The detection wavelength was at 260 nm. Results： Protocatechuic acid showed a good linear relationship at a range of 0.1032 - 0.516 μg, r = 0.99996. The average recovery was 97.27%, RSD = 1.4%. Conclusion： The method is simple with good reproducibility and can be used as the quality control of Yinxie Granule.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2006年第3期381-383,共3页
Chinese Journal of Pharmaceutical Analysis
关键词
银屑冲剂
高效液相色谱法
土茯苓
拔葜
原儿茶酸
Yinxie Granules
HPLC
rhizoma smilacis glabrae、smilax China L.
protocatechuic acid
