高效液相色谱法测定华细辛不同部位马兜铃酸A含量

Quantitative analysis of aristolochic acid A in different parts of Asarum sieboldii Miq.bv HPLC

目的:检测细辛药材不同用药部位。肾毒性成分马兜铃酸 A 的含量。方法:高效液相色谱方法,采用 Agilent Zorbax SB-C_(18)色谱柱(4.6 mm×250 mm,5μm),以乙腈-1%冰醋酸水溶液(39:61)为流动相,流速为0.8 mL·min^(-1),检测波长为317 nm,分别测定细辛全草和根、芦头、叶柄、叶片、花5个部分马兜铃酸 A 的含量。结果:高效液相色谱方法的回收率为98.7%,马兜铃酸 A 进样量在5.2～68ng 范围内线性关系良好;细辛全草含马兜铃酸 A 16.2μg·g^(-1),根和芦头中含微量马兜铃酸 A,叶柄和叶片中有较多马兜铃酸 A,花中含量最高。结论:细辛地上部分和地下部分马兜铃酸 A 含量差别很大。

Objective： To determine the nephropathy - causing substance, aristolochic acid A, in different parts of Asarum sieboldii Miq. Methods： An HPLC method was established with a Agilent Zorbax SB -C18 column （4.6 mm×250 mm, 5 μm）, using a mobile phase of acetonitrile -0.1% acetic acid solution （39：61）, a flow rate of 0.8 mL·min^-1, a detection wavelength of 317 nm to determine the contents of aristolochic acid A in Asarum sieboldii Miq. ＇s root, roothead, petiole, lamina, flower and the whole plant. Results： The average recovery rate was 98.7%, and there was a good linearity from 5.2 ng to 68 ng. The concentration of aristolochic acid A in the whole plant was 16.2 μg·g^-1, and there were very little in the root and roothead, and more in petiole and lamina, and the most in flower. Conclusion： The underground parts of Asarum sieboldii Miq. have much aristolochic acid A while the root has very little, and the root is more suitable to be used in medicine.