海洋微藻对UV-B辐射的生理生化响应

The physiological and biochemical responses of marine microalgae to UV-B radiation

用生理生化的方法研究了UV-B辐射对海洋微藻———小新月菱形藻(Nitzschia closte-rium)生理生化的影响。结果表明:(1)随着UV-B暴露时间的延长,UV-B辐射对藻的生长以及光合速率有抑制作用,并且时间越长,抑制作用越明显;(2)UV-B辐射使小新月菱形藻可溶性糖含量降低,而且在最初的2d,UV-B辐射使可溶性糖含量降低迅速,第4天的可溶性糖含量稍有所升高但与第2天相比差异不显著,UV-B辐射第6天,可溶性糖含量又迅速降低;(3)UV-B辐射的可溶性蛋白含量在第2天明显升高,随后又快速下降;(4)对照组的还原型谷胱甘肽(GSH)含量的变化随时间延长而升高,UV-B辐射第2天的GSH含量比初始状态(0d)的GSH含量稍有升高,但变化不明显,第2天之后,GSH含量随时间延长而快速降低;(5)对照组的过氧化物酶(POD)活性随时间延长而升高,UV-B辐射第2天的POD活性比初始状态(0d)的POD活性显著升高,第2天之后,POD活性随时间延长而快速降低。

The physiological and biochemical responses of marine microalgae to UV-B irradiation were examined by using the physiological and biochemical methods in this article. The results showed that （1） UV-B irradiation can inhibit the growth and photosynthetic rate of Nitzschia closterium, and the inhibiting effects were more significant when the time was prolonged; （2） UV-B irradiation decreased soluble sugar. In the initial 2 days, soluble sugar of N. closterium decreased rapidly, the soluble sugar on the 4th day has no major change compared with the soluble sugar on the 2^nd day, on the 6^th day, the soluble sugar decreased again; （3） The protein content of UV-B treated group significantly increased on the 2^nd day, rapidly decreased subsequently; （4） GSH content of control group increased as time prolonged, the GSH content on the 2^nd day has no major change with initial time （0 d）, after the 2^nd day, the GSH content decreased rapidly; （5） POD activity of control group increased as time prolonged, the POD activity on the 2^nd day increased significantly compared with POD activity on the initial time （0 d）, after the 2^nd day, POD activity rapidly decreased as time prolonged.