中华绒螯蟹不同组织LDH同工酶凝胶电泳分析

Gel electrophoresis of lactate dehydrogenase(LDH)isozymes in different tissues of Eriocheir sinensis

采用高pH不连续系统聚丙烯酰胺垂直板凝胶电泳,特异性组织化学染色对中华绒螯蟹(Eriocheir sinensis)的血清、肌肉、鳃、心脏、肝胰腺、精巢和精子等组织的乳酸脱氢酶(LDH)同工酶酶谱进行了研究。同时,利用2种底物(乳酸钠和α-羟基丁酸钠)进行了特异性反应,并对其精子特异性乳酸脱氢酶进行了研究。结果表明:以乳酸钠为底物时,中华绒螯蟹的肌肉、鳃、血清和心脏有2条谱带,而精巢和精子有3条谱带,但在肝胰腺中未发现谱带;以α-羟基丁酸钠为底物时,只在血清、精巢和精子发现有1条谱带。从而说明,中华绒螯蟹乳酸脱氢酶同工酶存在组织特异性;精子存在特异性乳酸脱氢酶。

The electrophoretograms of lactate dehydrogenase isozymes were studied in several tissues of Eriocheir sinensis by the high pH discontinuous system of polyacrylamide gel electrophoresis（PAGE）and the specific histochemical staining. At the same time, the two specific substrates （sodium lactate and α-Hydroxybutyric acid sodium salt）were managed in a specific reaction, and sperm-specific LDH was researched by the specific dyeing. When sodium lactate served as the substrate in the muscle, gill, serum and heart, it was observed that the electrophoretograms of LDH were consisted of 2 bands, in the testis and sperm, there were 3 bands, in the hepatopancreas, however, there was no band. When α-Hydroxybutyric acid sodinm salt served as the substrate in serum, testis and sperm, it was observed that the electrophoretograms of LDH were consisted of one band. The results indicated that LDH isozymes had tissue specificity, the sperm of E. sinensis had sperm-specific LDH.