双液相萃取菜籽粕蛋白提取工艺条件的选择

Effects of Some Extraction Conditions on Protein Extractability of the Two-phase Extracted Rapeseed Meal

以宁油七号双液相萃取菜籽粕为研究对象，采用正交试验和单参数条件试验相结合的方法，就菜籽分离蛋白提取过程的有关工艺参数进行了研究。试验表明，在一定的ｐＨ值下，温度是影响菜籽蛋白萃取率的最显著因素。在保证良好搅拌的条件下，菜籽蛋白萃取过程表现为速率控制过程，水粕比对蛋白萃取率影响不大，而萃取时间对菜籽蛋白萃取率有显著影响。宁油七号双液相萃取粕蛋白质的最佳萃取工艺参数，宜选取温度４０℃，水粕比１５，时间６０ｍｉｎ，ｐＨ＝１２。在该条件下菜籽蛋白的萃取率为７１．５％，植酸萃取率为３３．６％。

The effects of pH, the solvent-to-meal ratio(R), the extraction time(t), and the temperature(T)on the protein extractabiligy of the two-phase extracted rapeseed meal were studied. The optimum conditions for the rapeseed protein extraction were PH=12, R=15, t=60min, and T=40℃.Over70% of the protein in the meal was extracted with a phytic acid extractability of about 34% under the optimum conditions.