乳化异氟烷延迟相预处理对缺血再灌注损伤兔心肌的作用

Emulsified isoflurane produces delayed preconditioning against myocardial ischemia and reperfusion injury in rabbits.

目的探讨乳化异氟烷静脉输注是否具有延迟相心肌保护的作用。方法30只兔随机分为三组,每组10只,分别给予生理盐水、脂肪乳、乳化异氟烷。各组在给药后24h建立缺血再灌注模型,采用红四氮唑染色法测定心肌梗死范围。采用末端标记技术(TUNEL)检测心肌凋亡指数,免疫组化方法(SABC)检测Bcl-2和Bax的蛋白表达。结果脂肪乳组心肌梗死范围(45.27%±3.03%)与对照组(44.32%±2.15%)相比无显著差异;乳化异氟烷组(26.76%±2.23%)显著低于对照组、脂肪乳组(P<0.01);乳化异氟烷组心肌凋亡明显减少(P<0.01),Bcl-2蛋白表达增加(P<0.01),Bax蛋白表达降低(P<0.01)。结论乳化异氟烷静脉输注具有降低缺血再灌注后心肌梗死范围和抗凋亡的作用。

Objective To investigate whether infuse emulsified isoflurane can induce a delayed cardioprotection against Ischemia and reperfusion injury in rabbits. Methods Thirty rabbits were randomly assigned to receive saline, fat emulsion and emulsified isoflurane. They were then underwent 30 min of left anterior descending coronary artery occlusion followed by 3 h of reperfusion 24 h latter. Myocardial infarct size measured using triphenyltetrazolium staining. TUNEL to examine apoptosis and Immunohistochemistry to examine Bcl-2 and Bax protein expression were performed in rabbit hearts. Results Fat emulsion did not affect infarct size （45. 27%± 3. 03%） but emulsified isoflurane produced a reduction in infarct size （26. 76% ± 2. 23% ） as compared with control （44. 32%±2. 15% ,P〈0. 01） and significantly decreased the apoptosis and Box protein expression but increased Bcl-2 protein expression（P〈0. 01）. Conclusion Emulsified isoflurane produces a delayed preconditioning against myocardial ischemia and reperfusion injury.