哮喘大鼠静脉血和支气管肺泡灌洗液中降钙素基因相关肽的研究

An experimental study of calcitonin gene-related peptide in venous blood and in bronchoalveolar lavage fluid in asthmatic rats.

目的观察大鼠哮喘模型静脉血和支气管肺泡灌洗液(BALF)中降钙素基因相关肽(CGRP)的变化及其在哮喘发病机制中的作用。方法用卵蛋白(OVA)致敏SD大鼠并雾化吸入刺激,制作大鼠哮喘模型。10只大鼠作为哮喘组,7只大鼠作为正常对照组,收集静脉血和BALF,肺组织做病理切片,BALF进行HE染色并细胞分类计数,采用ELISA法检测静脉血和BALF中CGRP的浓度。结果哮喘组BALF中可见大量嗜酸粒细胞,与对照组比较,有极显著性差异(P<0.01)。哮喘组静脉血中CGRP水平与对照组比较有极显著性差异(P<0.01);哮喘组BALF中CGRP水平与对照组比较,有显著性差异(P<0.05)。结论哮喘大鼠静脉血和BALF中CGRP均明显升高,CGRP在哮喘的发病机制中可能起着重要的作用。

Objective To observation the role of calcitonin gene-related peptide（CGRP） in the pathogenesy of asthma by an experimental study of CGRP in venous blood and in bronchoalveolar lavage fluid（BALF） in asthmatic rats. Methods SD rats were sensitized and challenged with ovalbumin to establish an asthmatic model. There were 10 rats as asthma tic group and 7 rats as control group. The venous blood and the BALF were collected. Pathological section of the lungs was made and cell composition in the BALF was detected by HE staining. The concentration of CGRP in blood and in BALF was assayed by ELISA. Results In the asthmatic group, the number of eosinophils in BALF was obviously increased than that in control group. There was significant difference between two groups （P〈0. 01）. In the asthmatic group, the concentration of CGRP in venous blood was obviously increased than that in control group. There was significant difference between two groups （P〈0. 01）. In the asthmatic group, the concentration of CGRP in BALF was obviously increased than that in control group. There was significant difference between two groups （P〈0.05）. Conclusion Both CGRP in venous blood and in BALF in asthmatic rats were obviously increased, CGRP may play a key role in the pathogenesy of asthma.