急性肝内胆汁淤积兔血清、胆汁SIgA的检测及意义

The alternation of SIgA in serum and bile in the rabbit-models of acute intrahepatic cholestasis.

目的探讨急性肝内胆汁淤积新生兔血清、胆汁中分泌性IgA(SIgA)的变化及意义。方法将39只新生大白兔随机分成3个实验组(每组9例)和对照组(12例)。实验组采用一次灌服α-萘异硫氰酸盐(200mg/kg)的方法造成急性肝内胆汁淤积。分别于造模后24、48、72h依次收集3个实验组动物标本,检测血清和胆汁中的SIgA含量。结果急性肝内胆汁淤积兔血清中SIgA在24h开始升高(P<0.05),48h和72h较对照组有更为明显的升高(P<0.01);实验组兔胆汁中的SIgA含量在各个时间点均极明显低于对照组(P<0.01)。结论急性肝内胆汁淤积有血清和胆汁中的SIgA含量的变化,与肝胆系统损伤严重性和病程有关。

Objective To study the alternation of SIgA in serum and bile in experimental acute intrahepatic cholestasis. Method 39 newborn rabbits were divided at random into four groups： 3 experimental groups （n = 9） and a contral group （n = 12）. A single dose （200 mg/kg） of ANIT（α-naphthyl isothiocyanate） was administered by garage to each experimental rabbit to induce intrahepatic cholestasis. The specimens of serum and bile were collected from rabbits at 24 h, 48 h and 72 h after intoxication and detected by SIgA radioimmunologic kit. Result Compared with the control group, the SIgA in serum of experimental groups began to increase 24 h after intoxication（P〈0. 05）, then were getting singnificantly higher（P〈0. 01 ）. And the concentration of bile SIgA decreased dramatically（P〈0. 01） . Conclusion Acute intrahepatic cholestasis induced by ANIT re sulted in alterations of SIgA in serum and bile, and the alternation may be used to estimate the injury of hepatobiliary system.