摘要
目的观察中药加味肾气汤对镉所致大鼠肾脏慢性损伤的保护作用。方法将24只SD大鼠随机平均分为4组,正常组饮用自来水,模型组、给药组和阳性对照组饲喂200 mg.L-1的氯化镉溶液,3周后给药组以27mL.kg-1的剂量灌胃给予中药加味肾气汤煎剂,阳性对照组灌胃给予亚硒酸钠0.05 mg.kg-1,模型组和正常组不给予任何药物。于实验第14周将大鼠脱臼处死,留取两侧肾脏,用流式细胞仪观察大鼠肾脏上皮细胞凋亡情况。结果给药组大鼠肾近曲小管上皮细胞凋亡率明显低于模型组和阳性对照组,且与正常组接近。结论加味肾气汤对镉所致大鼠肾脏上皮细胞凋亡有显著的抑制作用,效果优于亚硒酸钠,对镉所致肾脏慢性损伤有明显的保护作用。
Objective To test the function of a TCM proscription, jiaweishenqitang, to prevent chronic cadimiuminduced injury of kidney. Methods The proscription of TCM fiaweishenqitang was used to to alleviate the cadimium-induced chronic toxicity in kidney and its function of inhibiting apoptosis in renal tubular epithelial cells （RTEC） was observed throughflow cytometer. Results This prescription of TCM can markedly inhibit apoptosis in RTEC and its function is superior to that of Na2SeO3. Conclusion The prescription of TCM fiaweishenqitang exhibits a prominent protective action on kidney against the cadimium-induced chronic injury.
出处
《医药导报》
CAS
2006年第5期389-391,共3页
Herald of Medicine
关键词
加味肾气汤
镉
细胞凋亡
Jiaweishenqitang
Cadimium
Apoptosis
