浅层液体培养在生姜脱毒苗快繁体系中的应用

Application of Shallow Liquid Culture System for the Rapid Propagation Technology of Virus-free Ginger

对浅层液体培养与固体培养在生姜脱毒苗继代、生根及移栽过程中进行了比较研究;结果表明浅层液体培养继代增殖系数、生根长度及株高均优于固体培养;根数、生根率在两种培养方式中没有明显差异;来源于浅层液体培养生根的生姜脱毒苗移栽成活率明显高于来源于固体培养基的脱毒苗;使用浅层液体培养节约化学药品成本66.7%以上,同时减少大量用工;据此认为,浅层液体培养更适合于脱毒生姜种苗的大量繁殖。

This paper studied on the difference of a shallow liquid culture system and the solid culture system in the system of rapid propagation technology of virus-free ginger. The multiplication coefficient,the length of root and plant height in shallow liquid culture were prior to those of solid culture. As the regeneration frequency of root and the average root number of each explant were analyzed, there was no significant difference between the two systems. The survival rate of transplantation of seedlings from the shallow liquid system was much higher than that from solid culture system. Furthermore, the cost of chemicals of shallow liquid system was only less than 1/3 compared to that of solid system. Meanwhile, the load of manual work and time were reduced much more. So it was more economical and convenient. For all of the above, the shallow liquid culture system was proposed during rapid propagation process of virus-free ginger.