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小鼠胚胎干细胞培养条件的探索 被引量：1

Culture and Biological Characteristics of Mouse Embryonic Stem Cell
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摘要 目的：探讨小鼠胚胎干细胞（ES细胞）的培养特点及生物学特性，为ES细胞的应用研究奠定基础。方法：小鼠胚胎成纤维细胞原代培养，3～5代的细胞作为饲养层细胞培养ES细胞。另在无饲养层细胞、明胶包被培养皿、培养液中含白血病抑制因子条件下培养小鼠ES细胞，观察2种情况下ES细胞的生长情况。结果：小鼠胚胎成纤维细胞呈放射状或旋涡状走行，ES细胞在2种条件下均能呈克隆状生长，且保持未分化状态，碱性磷酸酶（AKP）染色呈阳性。结论：2种条件下ES细胞均能良好生长。 Objective： To investigate the culture and biological characteristics of mouse embryonic stem （ES） cell as a foundational work for its application. Methods： Mouse ES cells were observed in two culture systems： 1） on feeder cells of the primary mouse embryo fibroblasts and 2） in leukemia with inhibititory factor but without feeder cells. Results： The primary mouse embryo fibroblast growth trended radially or circinately. The ES cell clones under the two conditions appeared typical characteristics and maintained un-differentiation state. Conclusion： The ES cells can grow well under those conditions.
作者 黄璟 何志旭
机构地区 贵阳医学院附院儿科
出处 《贵阳医学院学报》 CAS 2006年第2期109-112,共4页 Journal of Guiyang Medical College
基金 贵州省卫生厅资助项目
关键词 干细胞 胚胎 细胞 培养的 小鼠 近交BALB C 胚胎干细胞 stem cells embryo cells cultured mice,inbred BALB C embryonic stem cell
分类号 R394-33 [医药卫生—医学遗传学] Q813.11 [生物学—生物工程]
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参考文献8

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同被引文献10

  • 1Evans M J, Kaufman MH. Establishment in culture of pluripotential stem cells from mouse embryos[J]. Nature, 1981,291:154-156.
  • 2Irene G, Luo YQ. Differences between human and mouse embryonic stem cells[J]. Developmental Biology, 2004:360-380.
  • 3Davor S, Knowles BB. Immunosurgery of mouse blastocyst , Immunology, 1975, 72(12):5099-5102.
  • 4Zhou H, Wu S, Joo JY, et al. Generation of Induced Pluripotent Stem Cells Using Recombinant Proteins[J]. Cell Stem Cell, 2009.04.005.
  • 5Kim HS, Son HY, Kim S, et al. Isolation and initial culture of porcine inner cell masses derived from invitro-produced blastocysts [J]. Zygote ,2007, 15:55-63.
  • 6Verma V, Gautam SK, Singh B, et al. Isolation and Characterization of Embryonic Stem Cell-Like Cells From In Vitro-Produced Buffalo (Bubalus bubalis) Embryos [J]. Molecula rreproduction and development, 2007, 74:520-529.
  • 7Byrnel JA, Pedersen DA, Clepper LL, et al. Producing primate embryonic stem cells by somatic cell nuclear transfer [J]. Nature, 2007, 450, 22:450:497-502.
  • 8Kitani H, Takagi N, Atsumi T. Isolation of a germline-trans-missible embryonic stem (ES) cell line from C3H/He mice[J]. Zool. Sci.,1996, 13:865-871.
  • 9高巍,刘福强,梅晰凡,刘畅,任甫.昆明种小鼠胚胎干细胞的培养及鉴定[J].辽宁医学院学报,2008,29(1):1-3. 被引量：1
  • 10刘默芳,王恩多.绿色荧光蛋白[J].生物化学与生物物理进展,2000,27(3):238-243. 被引量：27

引证文献1

贵阳医学院学报
2006年 第2期

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