柠檬酸化硫酸钙对鼠成骨细胞增殖、分化的影响

Effect of citrated calcium sulphate on osteoblasts proliferation and differentiation

目的:观察柠檬酸化硫酸钙对成骨细胞功能的影响,探讨该材料作为骨移植替代材料的可行性。方法:采用体外培养成骨细胞的方法,观察材料浸提液对成骨细胞增殖、碱性磷酸酶和骨钙素分泌能力的影响。结果:在100%浓度柠檬酸化硫酸钙浸提液中培养的成骨细胞增殖相对缓慢(但相对增殖率>90%),其细胞核内嗜银蛋白颗粒染色分析与对照组无显著性差异(P>0.05),其分泌骨钙素、碱性磷酸酶能力显著高于对照组(P<0.01)。结论:柠檬酸化硫酸钙对成骨细胞不具有细胞毒性作用,随着硫酸钙的溶解,在材料植入部位的局部形成高钙环境,这有利于成骨细胞的增殖和分化促进新骨的形成。

Objective ：To study the effects of Citrated Calcium Sulphate （CCaS） on osteoblasts function and explore the possibility of this biomaterial as bone graft substitutes. Methods： Using the method of in vitro cell culcure, the influences of CCaS on the osteoblasts functions, such as cell proliferation, ALP activity, osteocalcin production, were detected. Results：Osteoblasts cultured in DMEM containing 100% concentration CCaS solutions growed slightly slower, but their relatively growth rates （RGR） were superior to 90%. They had the same AgNOR number as in control group, and excreted much more ALP and osteocalcin compared to in control group. Conclusion：CCaS has no toxtic effects on osteoblasts and stimulates the proliferation and differenti- ation of osteoblasts.