不同胎龄人胎脑神经干细胞发育特征探讨

Investigation on developmental characters of neural stem cells in striatuma from human fetal brain at different age

目的：探讨不同胎龄胎脑纹状体神经干细胞的发育特征.方法：收集胎龄16～28周的水囊引产胎儿60例,采用免疫组织化学及光镜技术对人胎脑纹状体神经干细胞的分布、形态以及数量进行检测.结果：不同胎龄胎脑纹状体均存在神经干细胞,神经干细胞主要分布在豆状核和尾状核,内囊处较少,呈圆形、椭圆形、三角形及多角形细胞,以椭圆形细胞多见,细胞有0～3个突起,胞核呈圆形和椭圆形,1～4个核仁不等,大部分细胞染色质疏松,少部分细胞染色质致密.随着胎龄的增加,纹状体神经干细胞逐渐减少.结论：不同胎龄人胎脑纹状体神经干细胞在形态及数量上存在一定的差异,而在分布部位无明显差异.

Objective：To investigate the developmental characters of neural stem cells （NSCs） in striatuma from human fetal brain at different age. Methods：60 cases of embryoes at gestational age 16w -28w and by induction of labor with water bag were collected for determining distribution, shapes, growth modes and the number of NSCs in striatuma with immunohistochemical method. Results： NSCs existed in striatuma from human fetal brain at different age. NSCs mainly distributed in lenticula and cauda nucleus, less in inner vesica. NSCs existed in striatuma of different fetal age included round, oval, triangle and polygon cells, The oval NSCs were more than others. NSCs had enations from 0 to 3. Nucli submitted round and oval shapes, each having nucleoli from 1 to 4. Most NSCs had rarefaction chromatin, the smaller part hadpykno-chromatin. NSCs in striatuma gradually decreased with increase of age. Conclusions ：There are differences on shapes and the number of NSCs in striatuma from different gestational age, but, there are no differences on distribution of NSCs.