反相离子对高效液相色谱法测定大鼠缺血再灌注心肌ATP的含量

Determination of ATP in the rat heart with ischemia reperfusion by ion-pair reversed-phase HPLC

目的：研究大鼠心肌缺血再灌注损伤后三磷酸腺苷（ATP）的含量的变化.方法：应用反相离子对高效液相色谱法（HPLC）分离测定,流动相为 0.1 mmol/L KH2PO4＋8 mmol/L 氢氧化四丁铵,检测波长259nm.结果：ATP标准液与峰面积呈良好的线性关系（r=0.998）.重复测定不同浓度ATP标准液精密度（变异系数）1.91%～5.78%.回收率为97.25%～100.05%.结论：反相离子对高效液相色谱法可以准确测定大鼠心肌组织ATP的含量.

Objectlve：To study the change alteration of ATP in the rat heart with ischemia reperfusion injury by Ion-pair reversed-phase HPLC. Methods： Ion-pair reversed-phase HPLC methods was developed for determination of ATP. The solvent consist of 0. 1 mmol · L^-1 KH2PO4 ; 8 mmol · L^-1 tetrabutylammonium hydrogen. The separation was monitored at 259nm. Results：The calibration curve showed good linearity （ r = 0. 998 ） . The coefficients of variation （ C. V） were estimated from repeated determinations of standard ATP. The intraassay C. V varied from 1.91% to 5.78%. The recovery was between 97.25% and 100.05%. Conclusions：This methods is precise,it can be used for determination of ATP in the rat heart.