先兆子痫患者HLA-DQA1、-DQB1、-DPA1基因多态性

HLA-DQA1、-DQB1 and HLA-DPA1 Genotypes in preeclampsia

目的:探讨人类白细胞抗原HLA-DQ-A1、DQB1、DPA1基因多态性与先兆子痫发病的关系。方法:采用序列特异性引物技术(PCRSSP)对46例先兆子痫患者和105例正常孕妇及其新生儿进行HLA-DQ-DPA1等位基因分型。结果:所有标本共检出11种HLADQA1基因表型、16种HLADQB1基因表型、6种HLADPA1基因表型。先兆子痫患者HLA-DQ-B10301基因频率高于正常孕妇,差异有显著性(Pc=0.032,RR=2.43,AR=0.30),其余各基因表型频率两组比较差异均无显著性。结论:HLADQB10301基因可能是一种先兆子痫发病的易感基因。

Objective：To clarify whether there are susceptible HLA-DQ or HLA-DPB1 alleles in women with preeclampsia. Methods ：The HLA-DQ and HLA-DPA1 typing were performed using sequence-specific amplification（PCR-SSP） in 46 patients and 105 healthy pregnant women and their newborns. Results：The HLA-DQB1 ＊ 0301 alleles frequency was higher in patients with preeclampsia （30. 43% ） than in the tested group of healthy controls（ 16. 19% ） （Pc = 0. 032 ,RR = 2.43, AR = 0. 30 ）, the HLA-DQA1 and HLA- DPB1 alleles frequency were no signficiant difference between two groups, so did newborns. No difference was found in HLA-DQ or HLA-DPBlmaternal-fetal genetic sharing between them. Condusion： Results suggest that the presence of HLA-DQB1 ＊ 0301 alleles may increase the risk of preeclampsia.