喘可治注射液对人外周血单个核细胞Th1/Th2细胞因子谱的影响

Effects of CKZ on Th1/Th2 cytokine repertoire of human peripheral blood mononuclear cells

目的:通过研究喘可治注射液对人外周血单个核细胞(PBMCs)Th1/Th2细胞因子谱的影响,探讨喘可治注射液的免疫调节作用机制。方法:以流式微球分析(CBA)法检测不同处理情况下,人外周血单个核细胞分泌Th1(IFN-γ、TNF-α、IL-2)和Th2(IL-4、IL-6、IL-10)细胞因子水平。结果:健康人PBMCs体外培养12小时后,上清中细胞因子主要为TNFα和IL6,喘可治使Th1和Th2细胞因子全面升高;喘可治对PDB加离子霉素诱导的PBMCs分泌Th1和Th2细胞因子具有抑制作用,并能抑制流感病人异常升高的INF-γ、TNF-α、IL-6和IL-10分泌。结论:喘可治注射液上调健康人PBMCs分泌Th1和Th2细胞因子,对异常活化的PBMCs分泌的Th1和Th2细胞因子则具有下调作用。

Objective：To study the immune regulation mechanisms of CKZ by investigating its effects on Th1/Th2 cytokine repertoire of human peripheral blood mononuclear cells （PBMCs）. Methods ：The concentrations of Th1 （ IFN-γ, TNF-α, IL-2） and Th2 （IL-4,IL-6, IL-10） cytokines secreted by PBMCs were detected by means of cytometric bead array（CBA）. Results：Cytokines secreted by PBMCs from healthy persons were mainly TNF-α and IL-6 after 12 hours of culture, and both Th1 and Th2 cytokines were increased by CKZ. Secretion of both Th1 and Th2 cytokines stimulated by PDB plus ionomycin were inhibited by CKZ, and CKZ could also inhibit the abnormally increased secretion of INF-γ, TNF-α, IL-6 and IL-10 in patients with influenza. Conclusion：CKZ can up-regulate Th1 and Th2 cytokines secreted by PBMCs from healthy person, and down-regulate Th1 and Th2 cytokines secreted by abnormally activated PBMCs.