调节性T细胞介导异基因交配孕鼠对父方抗原同种反应性的影响

Effects of regulatory T cells on the alloresponse mediated by allogeneic pregnant mice to the paternal alloantigens

目的研究怀有同种异基因胚胎对母体对父方抗原同种反应性的影响及其相关机制。方法采用CFSE标记应答细胞、SNARF-1标记刺激细胞的单向混合淋巴细胞培养体系,对孕10.5d的同基因交配孕鼠BALB/c×BALB/c(syn-BALB/c)和异基因交配孕鼠BALB/c×C57(allo-BALB/c)对C57小鼠同种抗原的应答强度进行比较;流式细胞术检测CD4+CD25+T细胞在syn-BALB/c和allo-BALB/c各淋巴器官的比例变化,及其胞内Foxp3表达。结果孕10.5d的allo-BALB/c对C57同种抗原的应答强度明显低于syn-BALB/c,但将应答细胞中的CD4+CD25+T细胞去除后,应答强度反而高于syn-BALB/c。allo-BALB/c脾脏、淋巴结和外周血CD4+CD25+T细胞明显高于syn-BALB/c,这些细胞均表达高水平的Foxp3。结论异基因交配孕鼠对父方抗原的同种反应性较同基因交配孕鼠降低,这一现象很可能是由CD4+CD25+Treg介导的。

Objective To investigate the effects of carrying allogeneic concepti on the maternal to paternal alloresponse and it＇s underlying mechanisms.Methods An one-way mixed lymphocyte reaction system characterized by labeling responder cells with CFSE and stimulator cells with SNARF-1 was established to measure the alloresponse mediated by syngeneic （BALB/c x BALB/c） and allogeneic （BALB/c x C57） pregnant mice, called syn-BALB/c and allo-BALB/c respectively, to C57 alloantigens at 10.5 days post coitus. Proportion of CD4 ^＋ CD25^＋ T cells in lymphoid organs, as well as their intracellular Foxp3 expression were measured by flow cytometry. Results The alloresponse against C57 antigens in allo-BALB/c was weaker than that in syn-BALB/ c, but much stronger when CD4^＋ CD25^＋ T cells were depleted from the responder cell population. The CD4^＋ CD25^＋ T cells frequencies were much higher in spleen,lymph nodes and peripheral blood of alloBALB/c than that of syn-BALB/c, with high level of Foxp3 expressions detected in almost all the CD4^＋ CD25^＋ T cells. Condusion The allogeneic pregnant mice exhibit decreased alloresponse to the paternal antigens than syngeneic pregnant mice, which is mediated by CD4^＋CD25^＋ regulatory T cells.