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磷脂酰肌醇3激酶抑制剂体外对弱精子症患者精子运动参数的影响

Influence of LY294002 on Sperm Motility in Asthenozoospermia Patients in Vitro
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摘要 目的:研究磷脂酰肌醇3激酶(PI3K)的特异性抑制剂LY294002在体外对弱精子症患者精子运动参数的影响,并探讨其可能的作用机制。方法:弱精子症患者和精液参数正常男性各10例,手淫法留取精液,经上游优化处理后的精子与不同浓度的LY294002孵育10、30、60 m in后,采用计算机辅助精液分析系统检测精子的运动参数。结果:LY294002在体外能显著提高弱精子症患者精子的活动率、前向性运动精子百分率和直线运动速度、平均路径运动速度。结论:LY294002在体外显著增强弱精子症患者精子的运动功能。 Objective ： To investigate the effects of LY294002, a phosphatidylinositol 3-kinase inhibition, on sperm motility in asthenozoospermia patients in vitro, and further analyze the possible molecular mechanism. Methods ： Sperm aseptically obtained by masturbation and prepared by swim-up technique from 10 patients with asthenozoospermia and 10 healthy fertile men were incubated with different concentrations of LY294002. Measurements of motility were carried out at 10, 30 and 60 min in all specimens by CASA. Results： The sperm in asthenozoospermia patients treated with LY294002 showed a significant increase in sperm progressive motility, the percentage of motile ceils, VSL and VAP. Conclusion： LY294002 can enhance the motility of sperm in asthenozoospermia pa- tients in vitro.
作者 杨军 刘继红 龚小新 王涛 陈俊 陈智
机构地区 华中科技大学同济医学院附属同济医院泌尿外科
出处 《中华男科学杂志》 CAS CSCD 2006年第4期311-314,共4页 National Journal of Andrology
关键词 弱精子症 磷脂酰肌醇3激酶抑制剂 LY294002 精子运动参数 体外研究 asthenozoospermia phosphatidylinositol 3-kinase inhibitor LY294002 sperm motile parameter in vitro
分类号 R698.2 [医药卫生—泌尿科学] R983 [医药卫生—药品]
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参考文献13

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二级参考文献4

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  • 4李杨,刘继红.双丁酰环磷酸腺苷对人精子运动功能的影响[J].中华男科学杂志,2002,8(6):398-400. 被引量：3

共引文献5

中华男科学杂志
2006年 第4期

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