精浆抗精子抗体阳性不育患者顶体酶、一氧化氮合酶及超氧化物歧化酶活力变化的研究

Study of the Changes of Acrosomal Enzyme and Nitric Oxide Synthase,Superxide dismutase of Infertile Patients with Positive Antisperm Antibody in Seminal Plasma

目的:研究精浆抗精子抗体(AsAb)阳性对精子顶体酶、精浆一氧化氮合酶(NOS)及超氧化物歧化酶(SOD)活力的影响。方法:精浆AsAb阳性不育者40例,对照组为40例正常生育男性。通过吸光度变化分别计算顶体酶活力(BAEE/ADH联合法)、NOS活力(氧化还原反应)、SOD活力(黄嘌呤氧化酶法)。结果:精浆As-Ab阳性组与正常生育组比较,精子顶体酶活力明显降低(P<0.01),NOS活力明显升高(P<0.01),精浆中SOD活力明显降低(P<0.01)。结论:精浆AsAb阳性引起不育可能与精子顶体酶、精浆中SOD及NOS活力改变有关。

Objective： To study the impacts of positive antisperm antibody （AsAb） in seminal plasma on acrosomal enzyme activity, nitric oxide synthase（NOS） and superxide dismutase（SOD） levels of spermatozoa. Methods： Swatch from 40 infertile patients with positive AsAb in seminal plasma as experimental group, and 40 fertile men as control group. Acrosomal enzyme activity was detected by the BAEE/ADH unitive method, NOS was detected by the redoxreaction assay, and SOD level was measured xanthine oxidase method. Results： Compared with control group, acrosomal enzyme activity of spermatozoa of experimental group was significantly decreased （P 〈 0.01 ） , NOS activity was apparently increased （P 〈 0.01 ） , and SOD level in seminal plasma was markedly decreased （ P 〈 0.01 ）. Conclusion ： It may be possible that the positive AsAb in seminal plasma beget infertility through the changes of acrosomal enzyme of spermatozoa, SOD and NOS activitise in seminal plasma.