奥硝唑和培氟沙星不同配伍对牙周病原菌体外抗菌作用

Antibacterial Activities of Different Proportions of Ornidazole and Pefloxacin Against Periodontal Pathogen in Vitro.

目的：了解奥硝唑和培氟沙星不同比例配伍对牙周病原菌体外抑菌效果。方法：1）奥硝唑和培氟沙星各自分为6水平，测量两种药物不同比例水平混合后对牙龈卟啉单胞菌，具核梭杆菌，中间普氏菌，消化链球菌标准菌株的抑菌指标变化，通过统计检验找出奥硝唑和培氟沙星不同配伍抑菌指标的无差别比例范围，并找出合适比例。2）检测两种药物以及其合适的比例对厌氧菌标准菌株和42株临床分离的非厌氧菌的MIC和MIC50。结果：牙龈卟啉单胞菌，具核梭杆菌，中间普氏菌，消化链球菌对奥硝唑和培氟沙星不同配伍所测指标的无差别范围分别是6：1—1：2、6：1—2：3、6：1—2：3、6：1—1：1。结论：两种药物混合对细菌的抑菌效果以2：3为佳，但有待进一步动物实验和临床验证。

Objective： To understand antibacterial activities of different proportions of Omidasole and Pefloxacin against periodontal pathogen in vitro. Methods： 1 ） Omidasole and Pefloxacin are divided to six levels, in order to measure the changes of antihlastic indexes of different proportions of Omidazole and Pefloxacin against Pg ,Fn,Pi,and Peptostreptoeoecus spp. Statistical test is used to seek the indistinctive ranges of antibacterial activities of Omidazole and Pefloxacin against those four kinds of typical periodontal pathogens, and to find the suitable proportion. 2 ） To measure MIC and MIC50 of Omidazole and Pefloxacin and the most suitable proportion against all bacterias. Results： The ranges of indistinctive antibacterial activities for four kinds of typical periodontal pathogens are 6：1 ～ 1： 2,6： 1 ～ 2： 3,6： 1 ～ 2：3 and 6：1 ～ 1 ： 1. Conclusion： The proportion of 2：3 can inhibit anaerobic bacterium and aerobic bacteria effectively, however, animal experiment and clinical verification are needed.