整合素连接激酶mRNA的表达与喉鳞状细胞癌相关性研究

Expression of Integrin-linked kinase is closely correlated with laryngeal squamous cell carcinomas

目的:探讨整合素连接激酶(ILK)与喉鳞状细胞癌(LSCC)发生、发展的关系。方法:应用RT-PCR方法,检测64例LSCC标本(LSCC组)和10例正常喉黏膜组织(对照组)中ILK mRNA的表达。结果:LSCC组ILK mRNA表达(0.644±0.098)明显高于对照组(0.032±0.026),差异有统计学意义(P<0.05);ILK mRNA在有淋巴结转移组织中的表达(0.867±0.247)明显高于无淋巴结转移组织(0.389±0.199),差异有统计学意义(P<0.05)。随着LSCC临床T分期的发展,ILK mRNA的表达均相应升高,但相邻两组间差异无统计学意义(均P>0.05)。结论:ILK的高表达对LSCC的形成和发展起重要作用;ILK mRNA高表达与LSCC淋巴结转移密切相关,提示ILK可能成为预测LSCC转移和预后的生物学指标。

Objective：To study whether Integrin linked kinase （ILK） is involved in the development and progression of laryngeal squamous cell carcinomas （LSCC）. Method：We examined the expression of ILK in 64 LSCC （LSCC group）and 10 normal laryngeal mucosa （control group） using reverse transcription polymerase chain reaction （RT-PCR） and analyzed the relationship with clinic pathological parameters. Result： The expression of ILK mRNA was significantly higher in 64 LSCC tissue （0. 644±0. 098） than in normal laryngeal mucosa tissues （0. 032±0. 026, P〈0.05）. The expression of ILK mRNA in LSCC with the metastasis of lymph nodes （0. 867±0. 247） was significantly higher than those without metastasis （0. 389±0. 199, P 〈0.05）. With the development of clinical T--stages of LSCC, the expression of ILK mRNA in LSCC increases. There was no significant difference between different clinical stage groups （ P 〉0.05）. Conclusion： These results suggest that ILK may participate not only in LSCC but also in invasion and metastasis of LSCC. ILK might be a novel molecular marker for prognosticating metastases and prognosis of LSCC.