Effect of WeiJia on carbon tetrachloride induced chronic liver injury

瞄准：为了在长期的肝损伤使用上学习 WeiJia 的效果，四氯化碳(CCl (4 )) 劝诱了肝损伤动物模型。方法: 称 180-220g 的 Wistar 老鼠随机被划分成三个组：正常控制组(组 A ) ，导致的肝损伤控制组织的 CCl (4 )( 组 B ) 和有 WeiJia 处理的正式就职组织的 CCl (4 )( 组 C ) 。每个组由 14 只老鼠组成了。肝损坏和纤维变性被皮下注射在 3 mL/kg 体重在齐墩果油与 40% CCl (4 ) 导致两次为为组 B 和 C 老鼠的八个星期的一个星期而齐墩果油被用于组 A 老鼠。从第三个星期开始，组 C 老鼠也在 1.25 microg/kg 体重的剂量收到了 WeiJia 的每日的腹膜内注射。动物在第五个星期被牺牲(4 男性， 3 女性) ，并且第八个星期(4 男性， 3 女性) 分别地。纤维变性的度被测量并且为肝纤维变性和功能包括透明质酸的血清学的标记(哈) ，类型 IV 骨胶原(CIV ) ， gamma-glutamyl 转移酶(gamma-GT ) ，丙氨酸 aminotransferase (中高音) 和 aspartate aminotransferase (著名计算机生产厂商) 被决定。α平滑肌肌动朊(alpha-SMA ) 和原子抗原(PCNA ) 免疫组织化学也是的增殖的房间表现了。结果：当时， CCl (4 ) 正式就职在组 B 和组 C 老鼠导致了肝的损坏和纤维变性的开发与组 A 相比老鼠。在组 C 老鼠的 WeiJia 的处理能与组 B 老鼠相比显著地减少纤维变性条件。效果能在三星期处理以后被观察并且在八星期处理以后是更明显的。浆液哈，在八星期为组 C 老鼠的处理以后的 CIV ，中高音，著名计算机生产厂商和 gamma-GT 层次是 58+/-22 microg/L ( P【0.01 )， 57+/-21 microg/L ( P【0.01 )， 47+/-10 U/L ( P【0.01 )， 139+/-13 U/L ( P【0.05 )和 52+/-21 U/L ( P】0.05 )分别地，从导致的肝损伤控制组织的 CCl ( 4 )(组 B )类似于正常控制组(组 A )，却显著地不同。PCNA 的增加和在 alpha-SMA 表示水平的减少也被观察。结论：WeiJia 能改进肝功能和可能通过星形的房间活动的抑制的还原剂肝纤维变性。

AIM： To study the effect of WeiJia on chronic liver injury using carbon tetrachloride （CCh） induced liver injury animal model. METHODS： Wistar rats weighing 180-220g were randomly divided into three groups： normal control group （Group A）, CCh induced liver injury control group （Group B） and CCI4 induction with WeiJia treatment group （Group C）. Each group consisted of 14 rats. Liver damage and fibrosis was induced by subcutaneous injection with 40% CCh in olive oil at 3 mL/kg body weight twice a week for eight weeks for Groups B and C rats whereas olive oil was used for Group A rats. Starting from the third week, Group C rats also received daily intraperitoneal injection of Wei.lia at a dose of 1.25 μg/kg body weight. Animals were sacrificed at the fifth week （4 male, 3 female）, and eighth week （4 male, 3 female） respectively. Degree of fibrosis were measured and serological markers for liver fibrosis and function including hyaluronic acid （HA）, type Ⅳ collagen （CIV）, γ-glutamyl transferase （γ-GT）, alanine aminotransferase （ALT） and aspartate aminotransferase （AST） were determined. Alpha smooth muscle actin （α-SMA） and proliferating cell nuclear antigen （PCNA） immunohistochemistry were also performed. RESULTS： CCl4 induction led to the damage of liver and development of fibrosis in Group B and Group C rats when compared to Group A rats. The treatment of WeiJia in Group C rats could reduce the fibrosis condition significantly compared to Group B rats. The effect could be observed after three weeks of treatment and was more obvious after eight weeks of treatment. Serum HA, CIV,ALT, AST and γ-GT levels after eight weeks of treatment for Group C rats were 58±22 μg/L （P〈0.01）, 57±21 μg/L （P〈0.01）, 47±10 U/L （P〈0.01）, 139±13 U/L （P〈0.05） and 52±21 U/L （P〉0.05） respectively, similar to normal control group （Group A）, but significantly different from CCh induced liver injury control group （Group B）. An increase in PCNA and decrease in α-SMA expression level was also observed. CONCLUSION： WeiJia could improve liver function and reduce liver fibrosis which might be through the inhibition of stellate cell activity.