系统性白念珠菌感染小鼠IL-12和IL-23的表达

The expression of IL-12 and IL-23 in mice with systemic Candida albicans infection

目的了解系统性白念珠菌感染小鼠IL-12和IL-23的表达特征,探讨IL-23在抗白念珠菌系统感染中的作用,并重新认识和评价IL-12的作用。方法通过尾静脉接种白念珠菌建立小鼠白念珠菌系统感染模型,观察小鼠肾脏的病理变化,RT-PCR法检测小鼠肾脏IL-23及IL-12mRNA的相对表达水平,平皿稀释法检测肾脏内菌落形成单位(CFU)。结果IL-12mRNA表达水平在初次感染后第1、3天明显升高(P<0.05),感染后第7天基本正常。IL-23mRNA水平在初次感染后的第1天无明显改变(P>0.05),感染后第3、7天明显升高(P<0.05),而再次感染中其mRNA始终呈现高表达(P<0.05)。结论IL-12和IL-23均参与I型免疫反应,有效抵御白念珠菌系统感染,IL-12可能在炎症反应的早期发挥重要作用,而IL-23可能在炎症反应的后期发挥重要作用。

Objective： To measure the expression of IL- 23 and IL - 12 in mice with disseminated eandidiasis, in order to understand the role of IL - 23 in the anti - systemic Candida albicans infection. Methods： The model of systemic Candida albicans infection was established by injection of the pathogenie fungi into the tail - vein of mouse to observe the pathological changes in the kidney of the mouse. RT - PCR was applied to detect the expression of IL -23 mRNA and IL- 12 mRNA in the kidney. Colony forming unit （CFU） of the infected kidney was determined with the plating dilution methods. Results： The level of IL- 12 mRNA was evidently higher than that of the normal controls on day 1 and day 3 （ P 〈 0.05）, and gradually returned to normal on day 7. In the primary infection the level of IL - 23 mRNA started to rise on day 3 and day 7, while the expression of IL - 23 mRNA rose to a high level from the beginning and persisted for the whole process, in the secondary infection （P〈0.05）. Conclusion： Both IL- 12 and IL - 23 are involved in type - I immune response and resist effectively against systemic Candida albicans infection. It is postulated that IL- 12 may play an important role in the early phase of the inflammation, while IL-23 lakes effect in the late phase.