Morphological observation of tumor infiltrating immunocytes in human rectal cancer

AIM： To investigate the morphological characterization of tumor infiltrating dendritic cells （TIDCs） and tumor infiltrating lymphocytes （TILs） in human rectal cancer. METHODS： Light and electron microscopy as well as immunohistochemistry were used to observe the distributive and morphological changes of TIDCs and TILs. RESULTS： TIDCs were mainly located in tumor-surrounding tissue. The number of TIDCs in the earlier stage was higher than that in the later stage （P〈 0.01）. TILs were mainly seen in adjacent tissue of cancers and tumor-surrounding tissue. There were more TILs in the earlier stage than that in the later stage （P〈0.01）. Under electron microscope, TIDCs were irregular in shape and exhibited many dendritic protrusions. It isn＇t obvious that cancer cells perforated the basement membrane and TILs were arranged along the basement membrane in the earlier stage. In the later stage, it is explicit that cancer cells perforated the basement membrane and surrounded by TILs. There were contacts among TIDCs, TILs and tumor cell. One IIDCs contacted one or several TILs which clustered around TIDCs. Glycogen granules were seen between TIDCs and Tits. CONCLUSION： The number of TIDCs and TILs is related with tumor progression There exist close relationships among TIDCs, TILs and tumor cell.

瞄准：调查渗入树枝状的房间(TIDC ) 的肿瘤和在人的直肠的癌症渗入淋巴细胞(TIL ) 的肿瘤的词法描述。方法：象免疫组织化学一样的光和电子显微镜学被用来观察 TIDC 和 TIL 的分发、词法的变化。结果：TIDC 主要位于包围肿瘤的织物。在更早的阶段的 TIDC 的数字在以后的舞台(P<0.01 ) 比那高。TIL 主要在癌症的邻近的织物和包围肿瘤的织物被看见。在以后的舞台(P<0.01 ) 比那在更早的阶段有更多的 TIL。在电子显微镜下面， TIDC 在形状是不规则的并且展出了许多树枝状的伸出。癌症房间穿孔了，不是明显的地下室膜和 TIL 在更早的阶段沿着地下室膜被安排。在以后的舞台，癌症房间穿孔了，是明确的地下室膜并且由 TIL 包围了。在 TIDC， TIL 和肿瘤房间之中有接触。一 TIDC 联系了在 TIDC 附近聚类的或几 TIL。肝糖小粒在 TIDC 和 TIL 之间被看见。结论：TIDC 和 TIL 的数字与在那里的肿瘤前进被联系近存在在 TIDC， TIL 和肿瘤房间之中的关系。