Effect of electro-acupuncture at Foot-Yangming Meridian on somatostatin and expression of somatostatin receptor genes in rabbits with gastric ulcer

瞄准：为了与胃溃疡在兔子在胃的粘膜损害， somatostatin (SS ) 和 SS 受体基因(SSR (1 ) mRNA ) 的表示上在 Foot-Yangming 顶点讨论电镀物品针灸的保护的效果并且推进，在基因表示水平探索顶点和内脏的相对特性。方法：四十只兔子随机被划分成控制组(A) ，胃溃疡模型组(B) ， Foot-Yangming 顶点组(C) ， Foot-Shaoyang 顶点组(D) 和 Foot-Taiyang 顶点组(E) 。胃溃疡模型被灌输酒精进胃准备。组 C-E 用分别地为 7 d 刺激仪器的顶点沿着上述顶点在点与电镀物品针灸被对待。在处理底，胃溃疡的索引被决定，表皮的生长因素(EGF ) 的数量和 somatostatin 被放射性免疫测定(RIA ) 测量。在胃粘膜的 SS-R (1 ) mRNA 表示被 RT-PCR 决定。结果：在模型组的 EGF 的值显然更低(73.6+/-14.8 对 91.3+/-14.9 pg/mL， P【0.01 ) 比那在控制组。胃溃疡的索引， SS 的内容和在胃粘膜的 SSR1mRNA 的表示比在控制组的那些显著地高(24.88+/-6.29 对 8.50+/-2.98 分数， P【0.01；2978.6+/-587.6 对 1852.4+/-361.7 mIU/mL， P【0.01；2.56+/-0.25 对 1.04+/-0.36， P【0.01 ) 。在 Foot-Yangming 顶点组的 EGF 的价值在模型组比那高(92.2+/-6.7 对 73.6+/-14.8 pg/mL， P【0.01 ) 。胃溃疡的索引， SS 的内容和在胃粘膜的 SS-R (1 ) mRNA 的表示是比在控制组的那些显著地低的(10.88+/-3.23 对 24.88+/-6.29 分数， P【0.01；1800.2+/-488 对 2978.6+/-587.6 mIU/mL， P【0.01；1.07+/-0.08 对 2.56+/-0.25 mIU/mL， P【0.01 ) 。比作模型组， SS 的内容和在在 Foot-Shaoyang 顶点组的胃粘膜的 SSR1mRNA 的表示减少了(2441.0+/-488 .vs 2978.6+/-587.6 mIU/mL， P【0.05； 1.73+/-0.16 对 2.56+/-0.25 mIU/mL， P【0.01 ) 。但是在 Foot-Taiyang 顶点组的上述参数没改善并且与在 Foot-Yangming 顶点组(P【0.05 ) 的那些显著地不同。结论：在 Foot-Yangming 顶点的电镀物品针灸能保护胃粘膜免于损害。机制可能与大脑勇气肽的规定和 SSR (1 ) mRNA 的表示有关。

AIM： To discuss the protective effect of electroacupuncture at the Foot-Yangming Meridian on gastric mucosal lesion, somatostatin （SS） and the expression of SS receptor genes （SSR1mRNA） in rabbits with gastric ulcer and to further explore the relative specificity of meridians and viscera at gene expression level. METHODS： Forty rabbits were randomly divided into control group （A）, gastric ulcer model group （B）, FootYangming Meridian group （C）, Foot-Shaoyang Meridian group （D） and Foot-Taiyang Meridian group （E）. The gastric ulcer model was prepared by infusing alcohol into stomach. Groups C-E were treated with electroacupuncture at points along the above meridians using meridian stimulating instruments for 7 days respectively. By the end of treatment, the index of gastric ulcer was determined, the amount of epidermal growth factor（EGF） and somatostatin was measured by radioimmunoassay （PJA）. SS-R1mRNA expression in gastric mucosa was determined by RT-PCR. RESULTS： The value of EGF in model group was obviously lower（73.6±14.8 vs 91.3±14.9 pg/mL, P〈 0.01） than that in control group. The index of gastric ulcer, content of SS and expression of SSRtmRNA in gastric mucosa were significantly higher than those in control group （24.88 ± 6.29 vs 8.50 ± 2.98 scores, P〈 0.01; 2978.6 ± 587.6 vs 1852.4 ± 361.7 mIU/mL, P〈 0.01; 2.56±0.25 vs 1.04±0.36, P〈0.01） . The value of EGF in Foot-Yangming Meridian group was higher than that in model group（92.2±6.7 vs 73.6±14.8 pg/mL, P〈 0.01）. The index of gastric ulcer, content of SS and expression of SS-R1mRNA in gastric mucosa were significantlylower than those in control group（10.88±3.23 vs 24.88±6.29 scores, P〈0.01; 1800.2±488 vs 2978.6±587.6 mIU/mL, P 〈 0.01; 1.07±0.08 vs 2.56±0.25mIU/mL, P〈0.01）. Compared to the model group, the content of SS and expression of SSRlmRNA in gastric mucosa in Foot-Shaoyang Meridian group decreased （2441.0±488. vs 2978.6± 587.6 mIU/mL, P〈 0.05; 1.73±0.16 vs 2.56±0.25 mIU/mL, P〈0.01）. But the above parameters in Foot-Taiyang Meridian group did not improve and were significantly different from those in Footoyangming Meridian group （P〈0.05） CONCLUSION： Electro-acupuncture at Foot-Yangming Meridian can protect gastric mocusa against injury. The mechanism may be releted to the regulation of brain-gut peptides and the expression of SSRtmRNA.