PCR检测急性脑炎病人脑脊液疱疹病毒DNA

PCR amplification of herpesvirus DNA in cerebrospinal fluid from patients with acute encephalitis.

本研究选用一对通用疱疹病毒属 DNA 多聚酶基因高保守区寡核苷酸引物作脑脊液标本DNA 多聚酶链反应(PCR),扩增产物作 SmaI 酶切分析,区分单纯疱疹病毒Ⅰ、Ⅱ型(HSV_1、HSV_2)、EB病毒(EBV)、巨细胞病毒(MCV)。结果46例成年临床疑诊急性病毒性脑炎病人中的21例(45.7%)脑脊液中检测出疱疹病毒 DNA 而得到确诊,其中 HSV_1 16例、HSV_2 3例、MCV 2例,未发现 EBV 阳性。结果提示,通用引物 PCR 是诊断急性疱疹病毒性脑炎的有效方法。

A single pair of oligonucleotide primers selected within a highly conserved region of the DNA polymerase of the herpesviruses was designed to amplify related viral genomes in cerebrospinal fluid,i,e.,herpes simplex virus type 1,type 2,Esptain-Barr virus, and cytomegalovirus(HSV_1,HSV_2,EBV,MCV),by polymerase chain reaction.A simple re- striction enzyme SmalⅠanalysis of these amplified products allowed accurate characteriza- tion of the herpesvirus type.Twenty-one cases of 46(45.7%)adult patients with suspected acute viral encephalitis were confirmed with the presence of herpesvirus DNA in cere- brospinal fluid,including 16 cases of HSV_1,3 cases of HSV_2,and 2 cases of MCV.Our re- sults suggests that polymerase chain reaction whith universal primers provides an effcient tecnique for the diagnosis of acute herpesvirus encephalitis.